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STEMCELL Technologies Inc stemspan sfemii
Stemspan Sfemii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+sfemii/stemspan+sfemii/pmc12256266-31-17-19
Average 90 stars, based on 1 article reviews
stemspan sfemii - by Bioz Stars, 2026-10
90/100 stars

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Recombinant:

Article Title: Humanized antibodies against c-Kit
Article Snippet: .. Frozen cord blood CD34+ stem/progenitor cells (ALLCELLS Catalog #CB005F) were resuspended in HSC retention media, StemSpan SFEMII (STEMCELL technologies Catalog #09605) supplemented with 20 ng/ml of human recombinant SCF (STEMCELL technologies Cat #78062), 20 ng/ml of Recombinant Human Flt3-Ligand (Peprotech Catalog #300-19) and 20 ng/ml of Recombinant Human TPO (Peprotech Catalog #300-18). .. About 3000 stem cells were plated per well on three COSTAR Ultra low cluster 96-well plates (Corning Catalog #7007).


Isolation:

Article Title: Expansion of human megakaryocyte-biased hematopoietic stem cells by biomimetic Microniche
Article Snippet: .. For the freshly isolated CD34 + cells from UCB or MNCs from UCB, PB, and BM, 2 × 10 5 initial seeding cells/well (24-well plate; Corning, 3527) were cultured in a medium composed of StemSpan SFEMII (STEMCELL Technologies), 1% penicillin/streptomycin (P/S; Sigma-Aldrich), 10 ng/ml human stem cell factor (hSCF; PeproTech), and 100 ng/ml human thrombopoietin (hTPO; PeproTech). .. The cultures were performed in a 2 ml medium/well supplemented with or without 1 μM SR1 (Alichem, 41864), 40 nM UM171 (APExBIO, A8950), 0.1% PVA (Sigma, P8136-250G), 4 mg/well Cytopore1 (GE Healthcare, 17-0911-01), 10 mg/well Cytodex3 (GE Healthcare, 17-0485-01), 10 mg/well Gelatin (CytoNiche), 10 mg/well Microcarrier W01 (CytoNiche), or 10 mg/well Microniche (CytoNiche, F01-50).

Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells.
Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells
Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

Cell Culture:

Article Title: Expansion of human megakaryocyte-biased hematopoietic stem cells by biomimetic Microniche
Article Snippet: .. For the freshly isolated CD34 + cells from UCB or MNCs from UCB, PB, and BM, 2 × 10 5 initial seeding cells/well (24-well plate; Corning, 3527) were cultured in a medium composed of StemSpan SFEMII (STEMCELL Technologies), 1% penicillin/streptomycin (P/S; Sigma-Aldrich), 10 ng/ml human stem cell factor (hSCF; PeproTech), and 100 ng/ml human thrombopoietin (hTPO; PeproTech). .. The cultures were performed in a 2 ml medium/well supplemented with or without 1 μM SR1 (Alichem, 41864), 40 nM UM171 (APExBIO, A8950), 0.1% PVA (Sigma, P8136-250G), 4 mg/well Cytopore1 (GE Healthcare, 17-0911-01), 10 mg/well Cytodex3 (GE Healthcare, 17-0485-01), 10 mg/well Gelatin (CytoNiche), 10 mg/well Microcarrier W01 (CytoNiche), or 10 mg/well Microniche (CytoNiche, F01-50).

Article Title: Engineering synthetic signaling receptors to enable erythropoietin-free erythropoiesis
Article Snippet: CD34 + HSPCs were sourced from fresh cord blood (generously provided by the Stanford Binns Family Program for Cord Blood Research) and Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA or STEMCELL Technologies, Vancouver, Canada). .. CD34 + HSPCs were cultured at 1-5×10 5 cells/mL in StemSpan SFEMII (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM, CellGenix, Freiburg, Germany) base medium supplemented with a human cytokine (PeproTech, Rocky Hill, NJ, USA) cocktail: stem cell factor (100 ng/mL), thrombopoietin (100 ng/mL), Fms-like tyrosine kinase 3 ligand (100 ng/ml), interleukin-6 (100 ng/mL), streptomycin (20 mg/mL) (ThermoFisher Scientific, Waltham, MA, USA), and penicillin (20 U/mL) (ThermoFisher Scientific, Waltham, MA, USA), and 35 nM of UM171 (cat.: A89505; APExBIO, Houston, TX, USA). ..

Article Title: Engineering synthetic signaling receptors to enable erythropoietin-free erythropoiesis.
Article Snippet: CD34+ HSPCs were sourced from fresh cord blood (generously provided by the Stanford Binns Family Program for Cord Blood Research) and Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA or STEMCELL Technologies, Vancouver, Canada). .. CD34+ HSPCs were cultured at 1-5×105 cells/mL in StemSpan SFEMII (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM, CellGenix, Freiburg, Germany) base medium supplementedwith a human cytokine (PeproTech, RockyHill, NJ, USA) cocktail: stem cell factor (100 ng/mL), thrombopoietin (100 ng/mL), Fms-like tyrosine kinase 3 ligand (100 ng/ml), interleukin-6 (100 ng/ mL), streptomycin (20mg/mL) (ThermoFisher Scientific, Waltham, MA,USA), andpenicillin (20U/mL) (ThermoFisher Scientific,Waltham, MA, USA), and 35 nM of UM171 (cat.: A89505; APExBIO, Houston, TX, USA). ..

Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells.
Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells
Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

Purification:

Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells.
Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells
Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

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